runx 2  (Cell Signaling Technology Inc)

Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences

Article Title: Combination of transcatheter arterial chemoembolization and anti-PD-L1 liposomes therapy suppressed hepatocellular carcinoma progression in mice.

doi: 10.1016/j.ejps.2023.106549

Figure Lengend Snippet: Fig. 5. The protein TGFβR2 located in the cellular membrane, while, the protein PTPN14 was mainly located in the nucleus (A). The mean fluorescence intensity of protein TGFβR2 in the normal control mice compared with the HCC model groups with or without treatments, &P<0.01 (B); the mean fluorescence intensity of protein PTPN14 in HCC model groups with liposome drug treatment compared with other groups, #P<0.05 (B), data were expressed as means ± SD, statistical significances were analyzed with one way ANOVA method.

Article Snippet: The proteins were extracted from the hepatic carcinoma tissues with RIPA Lysis Buffer containing with PMSF (catlog: N8031, Solarbio), then the protein concentration were analyzed with BCA Protein Assay Kit (catlog:PA115–01, TIANGEN), later, the protein were denaturated with hot water (80 ◦C-100 ◦C) for 1 hour, afterwards, the protein (15μl) was added into each hole of the isolated gels for isolating the proteins, afterthat, the protein gels were transferred to the PVDF membrane (Millipore, USA), then they were incubated in skim milk for 1 hour, later, washed with TBST buffer for three times, subsequently, the primary antibodies, TGFβR2 (catlog: ab186838, Abcam), SMAD7 (catlog: ab216428, Abcam), PTPN14 (catlog: 56612T, CST) and β-Actin (catlog: ab179467, Abcam) were used for the protein bands incubation overnight, besides, the protein β-Actin was a reference control protein.

Techniques: Membrane, Fluorescence, Control

Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences

Article Title: Combination of transcatheter arterial chemoembolization and anti-PD-L1 liposomes therapy suppressed hepatocellular carcinoma progression in mice.

doi: 10.1016/j.ejps.2023.106549

Figure Lengend Snippet: Fig. 4. Western blot detected the expression of proteins TGFβR2, SMAD7 and PTPN14 (A); TACE therapy and avelumab liposome drug therapy reduced the expression of TGFβR2 protein and up-regulated the expression of SMAD7 and PTPN14, ** P<0.05 of TGFβR2 protein in the TACE along with avelumab liposome drug therapy group compared with the other groups; * P<0.05 of SMAD7 protein compared with the hepatoma mice model group with TACE along with avelumab liposome drug therapy group; *** P<0.05 of PTPN14 protein compared with the hepatoma mice model group with TACE therapy or along with avelumab liposome drug therapy; #P<0.05 of PTPN14 protein compared with the hepatoma mice model group with TACE and avelumab liposome drug therapy. Data were expressed as means ± SD (B).

Article Snippet: The proteins were extracted from the hepatic carcinoma tissues with RIPA Lysis Buffer containing with PMSF (catlog: N8031, Solarbio), then the protein concentration were analyzed with BCA Protein Assay Kit (catlog:PA115–01, TIANGEN), later, the protein were denaturated with hot water (80 ◦C-100 ◦C) for 1 hour, afterwards, the protein (15μl) was added into each hole of the isolated gels for isolating the proteins, afterthat, the protein gels were transferred to the PVDF membrane (Millipore, USA), then they were incubated in skim milk for 1 hour, later, washed with TBST buffer for three times, subsequently, the primary antibodies, TGFβR2 (catlog: ab186838, Abcam), SMAD7 (catlog: ab216428, Abcam), PTPN14 (catlog: 56612T, CST) and β-Actin (catlog: ab179467, Abcam) were used for the protein bands incubation overnight, besides, the protein β-Actin was a reference control protein.

Techniques: Western Blot, Expressing